I did backfills on two Melibe brains. Both appeared very nicely.
My success rate on backfilling is pretty high, almost 100%.
It is simple. All you have to do is to let dye solution touch only the cut end of the nerve you want to fill. And make sure the prep is in healthy condition until fixation. Poor results are mostly due to failure in these two things. It is not because of materials or protocols. It is how you manipulate them.
No comments:
Post a Comment