Two serotonin blockers in Aplysia

cinanserin, methiothepin, cyproheptadine, spiperone.

10 mM 5-HT stock solutions were made in 0.1 M acetic acid solutions and stored at 4℃.

methiothepine blocked AC-coupled 5-HT receptors.
spiperone (100microM) blocks PKC-dependent modulatory effects of 5-HT, which is the facilitation from the depressed synapse.
spiperone is a selective antagonist of PLC-coupled 5-HT receptors in Aplysia.

Staurosporine is the PKC blocker.


Serotonin Receptor Antagonists Discriminate Between PKA- and PKC-Mediated Plasticity in Aplysia Sensory Neurons
Bogdan Dumitriu, Jonathan E. Cohen, Qin Wan, Andreea M. Negroiu and Thomas W. Abrams
J Neurophysiol 95: 2713-2720, 2006. First published October 19, 2005; doi:10.1152/jn.00642.2005