It has been postulated that vesicle mobility is increased to enhance release of transmitters, its mechanism was unknown.
Ryanodine receptor mediated Ca2+-induced Ca2+ release from ER is necessary for the sustained DCV mobilization.
This RyR-receptor mediated release is short-lived, and triggers activation of CaMKII as a downstream activator.
ryanodine (high purity) and other chemicals were purchased from Calbiochem.
4-bromo-clacimycin is a Ca2+ ionophore.
cytosolic calcium sensor, Cameleon 2.1.
CaMKII activity can be blocked by a membrane-permiable version of AIP-II (Antennapedia peptide fused).
veratridine, a sodium channel opener (that results in an increase of intracellular Ca2+).
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